| Property |
Value |
| Working Groups |
RG Flügel, 
RG Odoardi |
| Subproject |
A06 |
| Open Access |
Yes |
| Publication Type |
Journal Article |
| Peer Reviewed |
Yes |
| DOI |
10.1021/acsnano.6c08378 |
| Publication Year |
2026 |
| Title |
Nanobodies Equippedwith HaloTag Enable Scalable Fluorescence-LifetimeMultiplexing |
| Journal |
ACS Nano |
| ISSN |
1936-0851 |
| eISSN |
1936-086x |
| URL |
https://doi.org/10.1021/acsnano.6c08378 |
| Journal Abbreviation |
ACS Nano |
| Authors |
Albert L, Basak S, Körner H, Oleksiievets N, Mougios N, Cotroneo ER, Frei MS, Enderlein J, Broichhagen J, Simeth NA, Tsukanov R, Opazo F |
| First Author |
Albert L |
| Last Author |
Opazo F |
External Resources
pubs.acs...78.pdfhttps://pubs.acs.org/ancac3/article-pdf/doi/10.1021/acsnano.6c08378/68308132/acsnano.6c08378.pdf Article fulltext
2/180076https://publications.goettingen-research-online.de/handle/2/180076 GRO.publications identifier
9534https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9534 NCBI taxonomy (African green monkey, Chlorocebus aethiops)
10116https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=10116 NCBI taxonomy (rat, Rattus norvegicus)
0000-0003-4505-3010https://orcid.org/0000-0003-4505-3010 ORCID identifier (László Albert)
0000-0002-2645-1390https://orcid.org/0000-0002-2645-1390 ORCID identifier (Nazar Oleksiievets)
0009-0007-5712-7175https://orcid.org/0009-0007-5712-7175 ORCID identifier (Nikolaos Mougios)
0000-0003-0388-8626https://orcid.org/0000-0003-0388-8626 ORCID identifier (Elena Rebecca Cotroneo)
0000-0002-4799-4554https://orcid.org/0000-0002-4799-4554 ORCID identifier (Michelle Susanne Frei)
0000-0001-5091-7157https://orcid.org/0000-0001-5091-7157 ORCID identifier (Jörg Enderlein)
0000-0003-3084-6595https://orcid.org/0000-0003-3084-6595 ORCID identifier (Johannes Broichhagen)
0000-0001-8130-883Xhttps://orcid.org/0000-0001-8130-883X ORCID identifier (Nadja Anita Simeth)
0000-0002-3967-1755https://orcid.org/0000-0002-3967-1755 ORCID identifier (Roman Tsukanov)
0000-0002-4968-9713https://orcid.org/0000-0002-4968-9713 ORCID identifier (Luis Felipe Opazo Davila)
477https://sfb1286.uni-goettingen.de/literature/publications/477 Published Data Registry entry (CRC 1286)
23https://rdp.sfb1690.uni-goettingen.de/literature/publications/23 Published Data Registry entry (CRC 1690, preprint)
324https://rdp.sfb274.de/literature/publications/324 Published Data Registry entry (TRR 274)
1446https://prod.mbexc.uni-goettingen.de/literature/publications/1446 Published Data Registry entry (MBExC)
1/843https://mbexc.uni-goettingen.de/archive/1/843 Research Data Archive MBExC (supplemental material)
05a28rw58https://ror.org/05a28rw58 ROR identifier (05a28rw58, ETH Zurich)
010s54n03https://ror.org/010s54n03 ROR identifier (010s54n03, Leibniz-Forschungsinstitut für Molekulare Pharmakologie)
05xy1nn52https://ror.org/05xy1nn52 ROR identifier (05xy1nn52, Multiscale Bioimaging)
05591te55https://ror.org/05591te55 ROR identifier (05591te55, Ludwig-Maximilians-Universität München)
05b8d3w18https://ror.org/05b8d3w18 ROR identifier (05b8d3w18, Max Planck Institute of Molecular Cell Biology and Genetics)
021ft0n22https://ror.org/021ft0n22 ROR identifier (021ft0n22, University Medical Center Göttingen)
01y9bpm73https://ror.org/01y9bpm73 ROR identifier (01y9bpm73, University of Göttingen)
03xrhmk39https://ror.org/03xrhmk39 ROR identifier (03xrhmk39, Vlaams Instituut voor Biotechnologie)
AB_628437https://scicrunch.org/resolver/AB_628437 SciCrunch identifier (RRID:AB_628437)
AB_887862https://scicrunch.org/resolver/AB_887862 SciCrunch identifier (RRID:AB_887862)
AB_887875https://scicrunch.org/resolver/AB_887875 SciCrunch identifier (RRID:AB_887875)
AB_1843992https://scicrunch.org/resolver/AB_1843992 SciCrunch identifier (RRID:AB_1843992)
AB_1849446https://scicrunch.org/resolver/AB_1849446 SciCrunch identifier (RRID:AB_1849446)
AB_2083165https://scicrunch.org/resolver/AB_2083165 SciCrunch identifier (RRID:AB_2083165)
AB_2619883https://scicrunch.org/resolver/AB_2619883 SciCrunch identifier (RRID:AB_2619883)
AB_2620099https://scicrunch.org/resolver/AB_2620099 SciCrunch identifier (RRID:AB_2620099)
AB_2636902https://scicrunch.org/resolver/AB_2636902 SciCrunch identifier (RRID:AB_2636902)
AB_2921286https://scicrunch.org/resolver/AB_2921286 SciCrunch identifier (RRID:AB_2921286)
AB_3076022https://scicrunch.org/resolver/AB_3076022 SciCrunch identifier (RRID:AB_3076022)
AB_3076036https://scicrunch.org/resolver/AB_3076036 SciCrunch identifier (RRID:AB_3076036)
AB_3076052https://scicrunch.org/resolver/AB_3076052 SciCrunch identifier (RRID:AB_3076052)
AB_3076115https://scicrunch.org/resolver/AB_3076115 SciCrunch identifier (RRID:AB_3076115)
AB_3076116https://scicrunch.org/resolver/AB_3076116 SciCrunch identifier (RRID:AB_3076116)
AB_3076117https://scicrunch.org/resolver/AB_3076117 SciCrunch identifier (RRID:AB_3076117)
AB_3662033https://scicrunch.org/resolver/AB_3662033 SciCrunch identifier (RRID:AB_3662033)
AB_3668677https://scicrunch.org/resolver/AB_3668677 SciCrunch identifier (RRID:AB_3668677)
AB_3668678https://scicrunch.org/resolver/AB_3668678 SciCrunch identifier (RRID:AB_3668678)
AB_3668679https://scicrunch.org/resolver/AB_3668679 SciCrunch identifier (RRID:AB_3668679)
AB_3697322https://scicrunch.org/resolver/AB_3697322 SciCrunch identifier (RRID:AB_3697322)
AB_10672335https://scicrunch.org/resolver/AB_10672335 SciCrunch identifier (RRID:AB_10672335)
AB_10730034https://scicrunch.org/resolver/AB_10730034 SciCrunch identifier (RRID:AB_10730034)
CVCL_0224https://scicrunch.org/resolver/CVCL_0224 SciCrunch identifier (RRID:CVCL_0224, COS-7)
SCR_002630https://scicrunch.org/resolver/SCR_002630 SciCrunch identifier (RRID:SCR_002630, GitHub)
SCR_024664https://scicrunch.org/resolver/SCR_024664 SciCrunch identifier (RRID:SCR_024664, Leica: STELLARIS 8 microscope)
SCR_013673https://scicrunch.org/resolver/SCR_013673 SciCrunch identifier (RRID:SCR_013673, Leica Application Suite X)
SCR_001622https://scicrunch.org/resolver/SCR_001622 SciCrunch identifier (RRID:SCR_001622, MATLAB)
pubs.acs...i_001/https://pubs.acs.org/ancac3/article-supplement/5415226/pdf/nn6c08378_si_001/ Supplemental material
github.com/scstein/TrackNTracehttps://github.com/scstein/TrackNTrace Supplemental material (code, reused)
Linked Antibodies
| PID |
AG |
Type |
Antigen symbol |
Antibody Registry ID |
Name |
| primary-0049 |
|
primary |
VIM |
AB_628437 |
Vimentin Antibody (V9) |
| primary-0050 |
|
primary |
TUBA4A |
AB_887862 |
Anti-alpha-Tubulin antibody |
| primary-0051 |
|
primary |
SLC17A7 |
AB_887875 |
VGLUT 1 antibody |
| primary-0052 |
|
primary |
TOMM20 |
AB_1843992 |
Anti-TOMM20 antibody |
| primary-0053 |
|
primary |
GALNT2 |
AB_1849446 |
Anti-GALNT2 antibody produced in rabbit |
| primary-0054 |
|
primary |
CLTC |
AB_2083165 |
Anti-Clathrin heavy chain antibody |
| primary-0055 |
|
primary |
TH |
AB_2636902 |
Anti-Tyrosine hydroxylase antibody |
| primary-0056 |
|
primary |
GFAP |
AB_3076117 |
FluoTag®-X2 anti-GFAP Atto488 |
| primary-0057 |
|
primary |
PRVA |
AB_2619883 |
Parvalbumin antibody |
| primary-0058 |
|
primary |
SOX2 |
AB_2620099 |
SOX2 antibody |
| primary-0059 |
|
primary |
NUP50 |
AB_2921286 |
Anti-NUP50 antibody [EPR9526] |
| primary-0065 |
|
primary |
RBFOX3 |
AB_3662033 |
NeuN antibody |
| primary-0070 |
|
primary |
PMP70 |
AB_10672335 |
Anti-PMP70 antibody |
| primary-0071 |
|
primary |
Ctip2 |
AB_10730034 |
Anti-Ctip2 antibody [EPR23120-25] |
| secondary-0060 |
|
secondary |
|
AB_3076022 |
FluoTag®-X2 anti-Mouse IgG1 Atto643 |
| secondary-0061 |
|
secondary |
|
AB_3076036 |
FluoTag®-X2 anti-Rabbit IgG |
| secondary-0062 |
|
secondary |
|
AB_3076052 |
FluoTag®-X2 anti-Mouse IgG2a/b AlexaFluorTM 568 |
| secondary-0063 |
|
secondary |
|
AB_3076115 |
FluoTag®-X2 anti-GFAP AlexaFluorTM 568 |
| secondary-0064 |
|
secondary |
|
AB_3076116 |
FluoTag®-X2 anti-GFAP AlexaFluorTM 647 |
| secondary-0066 |
|
secondary |
|
AB_3668677 |
sdAb anti-Mouse IgG1 Halo fusion |
| secondary-0067 |
|
secondary |
|
AB_3668678 |
sdAb anti-Mouse IgG2a/b Halo fusion |
| secondary-0068 |
|
secondary |
|
AB_3668679 |
sdAb anti-Rabbit IgG Halo fusion |
| secondary-0069 |
|
secondary |
|
AB_3697322 |
FluoTag®-X2 anti-Rabbit IgG abberoir Star Green |